Differ icon 054e897f1aa9b61e853b7ee6ab0d3bf567857568a470a59bb7e255a869014a8c

Differ

browse, search, split and
combine VCFs


VCF was created by the 1000 Genomes Project as a way to store genome variations and has since become the de facto standard in human genomics. Handling VCF files, however, is not always straightforward. Although a VCF file can be opened and viewed in any text editor, browsing or searching whole genome VCF files is often slow and clumsy. Command-line tools designed to handle VCF files are faster, but are hard to use for non-bioinformaticians. Given the popularity of the VCF format, the genomics community is in need for an ultra-fast yet easy-to-use tool to browse, search, split and combine VCFs.

With Differ, Diploid delivers such a tool to the community, free of charge.

Blazingly fast 6965db5e3d16cb9eae857a4cb5f67ecd415a3071ed4b8cdecdfa7f7779d8e0c3

Blazingly fast

Opening a 1.5 GB VCF file in a text editor can take a while, even on modern computers. Differ opens these VCFs in a second or two on any modern Mac*. You’ll notice the difference.

* SSD recommended for optimal performance

01 572b0d3458148ec7e03d583e604b68882e37e818646d9a68534069ec330b27ff

No more 0/1

Differ presents variants in a table instead of showing them in plain text. Inspect the selected variant in a separate pane and see major properties at a glance. No more translating 0/1 either, Differ does the work for you and shows the corresponding alleles.

Multicore search 884ddc6f01743834524d98ee31f69bfa9668702aafd95887f0d56a5e8aa5ef78

Multicore search

The fastest way to search a VCF is through command-line tools, but to most geneticists using these tools feels like eating glass. Differ searches VCF files by dividing the search task over all available processor cores, allowing you to search VCFs faster than any bioinformatics guru - no Terminal required.

Snpeff 799522c299c7d1f12dd9f926e1336686d37eb51d23188977d7ee52cd9d213992

SnpEff aware

If your VCF has been annotated with SnpEff, Differ shows the SnpEff annotations in a separate table, allowing you to search variants by gene name or effect.

Export ba1321b977d02bbfebe7f33efbdc8239f41b07479a37bebc192475b7dbbbbc0b

Expert export

Focussing on a specific chromosome, gene or any other subset of variants? You can easily export variants of interest to Excel, Numbers or VCF.

Split 117525526556edefe159fd2df0fe3ba5c1bc1567f12d736b5f7a6b03f7ad78e7

Split VCFs in a split second

Need only specific samples in a multiplex VCF file? Simply select the samples you’re interested in and export them to separate files.

Better together 13b8e51d4a0731fb49f3568e735e08a486f2885619716e5d287b73e75b13f8cc

Better together

Until now, combining two VCFs into one was even harder than printing them and gluing them together. With Differ, you can combine two VCF files exactly the way you want.

Differ requires Mac OS X 10.10 Yosemite or later.